-
Recent Posts
- Appearance of iPSCs–Different Reprogramming Stages within the Same Well
- Picture Blog: A Short Path from Human mRNA-iPSCs to Neurons in Record Speed
- Picture Blog: Naive Human Pluripotent Stem Cells Regrown From Allele’s iPSCs
- Human Pluripotent Stem Cells Are Getting Naïve
- New Allele Biotech Publication on Stem Cells
Recent Comments
Archives
- March 2014
- February 2014
- December 2013
- November 2013
- October 2013
- September 2013
- August 2013
- July 2013
- June 2013
- May 2013
- March 2013
- January 2013
- December 2012
- November 2012
- October 2012
- September 2012
- August 2012
- July 2012
- April 2012
- March 2012
- February 2012
- January 2012
- December 2011
- November 2011
- October 2011
- September 2011
- July 2011
- June 2011
- May 2011
- April 2011
- March 2011
- February 2011
- January 2011
- December 2010
- November 2010
- October 2010
- September 2010
- August 2010
- July 2010
- June 2010
- May 2010
- April 2010
- March 2010
- February 2010
- January 2010
- December 2009
- November 2009
- October 2009
- September 2009
Categories
Meta
Tag Archives: shRNA lentivirus packaging
Top 10 List of Most Viewed AlleleBlogs in 2011
The ballot is in—among the “usual suspect” hot topics, iPS takes the top honor and most entries; Camelid antibodies, although not really presented as a typical AlleleBlog in 2011, made it to the top 3. shRNA cloning and RNAi screening … Continue reading
How do you make shRNA-expressing viruses for function screening?
Allele Weekly Blog: http://blog.allelebiotech.com/2011/11/how-do-you-make-shrna-expressing-viruses-for-function-screening/ Most people use standard cloning procedures when trying to insert shRNA templates into lentiviral vectors, i.e. anneal a pair of long oligos with sticky ends and ligate the dsDNA into a linearized plasmid with compatible overhangs. … Continue reading
Jiwu's space 